For the Round of 32, which takes place May 9-10, clubs have been split into eight groups.
Below, host clubs are listed first and MLS clubs are bolded.
Group 1
- Houston Dynamo FC vs. Sporting Kansas City – May 10, 8:30 pm ET
- Austin FC vs. New Mexico United (USL Championship) – May 10, 9 pm ET
Group 2
- Birmingham Legion FC (USL Championship) vs. Memphis 901 FC (USL Championship) – May 10, 8 pm ET
- Nashville SC vs. FC Dallas – May 10, 8:30 pm ET
Group 3
- LA Galaxy vs. Seattle Sounders FC – May 10, 10:30 pm ET
- Portland Timbers vs. Real Salt Lake – May 10, 10:30 pm ET
Group 4
- Sacramento Republic FC (USL Championship) vs. Colorado Rapids – May 9, 10:30 pm ET
- Monterey Bay FC (USL Championship) vs. LAFC – May 9, 10:30 pm ET
Group 5
- Loudoun United FC (USL Championship) vs. Columbus Crew – May 10, 7 pm ET
- FC Cincinnati vs. New York City FC – May 10, 7 pm ET
Group 6
- New York Red Bulls vs. D.C. United – May 9, 7:30 pm ET
- New England Revolution vs. Pittsburgh Riverhounds SC (USL Championship) – May 9, 7:30 pm ET
Group 7
- Charlotte FC vs. Orlando City SC – May 9, 7 pm ET
- Inter Miami CF vs. Charleston Battery (USL Championship) – May 9, 7 pm ET
Group 8
- Chicago Fire FC vs. St. Louis CITY SC – May 9, 8 pm ET
- Minnesota United FC vs. Philadelphia Union – May 9, 8:30 pm ET
Eight MLS teams – New York City FC, New York Red Bulls, Orlando City SC, Philadelphia Union, Austin FC, FC Dallas, LA Galaxy and LAFC – earned automatic Round of 32 spots based on last year’s standings. They will join 16 MLS teams that advanced via their Third Round results.
The tournament will culminate in a September 27 final, when a 2024 CONCACAF Champions League spot, prize money and silverware are all up for grabs for the victorious club.